Journal
JOURNAL OF BIOLOGICAL CHEMISTRY
Volume 287, Issue 42, Pages 35516-35526Publisher
ELSEVIER
DOI: 10.1074/jbc.M112.403253
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Funding
- United States Department of Energy [DE-AC02-06CH11357]
- Michigan Economic Development Corporation
- Michigan Technology Tri-Corridor Grant [085P1000817]
- National Institutes of Health from the United States Public Health Service [R01 ES010546, R01 ES010375, P01 ES05355, T32 ES007028, P30 ES000267]
- National Research Foundation from the Ministry of Education, Science and Technology Korea [2010-0006538]
- National Research Foundation of Korea [2010-0006538] Funding Source: Korea Institute of Science & Technology Information (KISTI), National Science & Technology Information Service (NTIS)
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N-2,3-Ethenoguanine (N-2,3-epsilon G)is one of the exocyclic DNA adducts produced by endogenous processes (e. g. lipid peroxidation) and exposure to bioactivated vinyl monomers such as vinyl chloride, which is a known human carcinogen. Existing studies exploring the miscoding potential of this lesion are quite indirect because of the lability of the glycosidic bond. We utilized a 2'-fluoro isostere approach to stabilize this lesion and synthesized oligonucleotides containing 2'-fluoro-N-2,3-epsilon-2'-deoxyarabinoguanosine to investigate the miscoding potential of N-2,3-epsilon G by Y-family human DNA polymerases (pols). In primer extension assays, pol eta and pol kappa replicated through N-2,3-epsilon G, whereas pol iota and REV1 yielded only 1-base incorporation. Steady-state kinetics revealed that dCTP incorporation is preferred opposite N-2,3-epsilon G with relative efficiencies in the order of pol kappa > REV1> pol eta approximate to pol iota, and dTTP misincorporation is the major miscoding event by all four Y-family human DNA pols. Pol iota had the highest dTTP misincorporation frequency (0.71) followed by pol eta (0.63). REV1 misincorporated dTTP and dGTP with much lower frequencies. Crystal structures of pol iota with N-2,3-epsilon G paired to dCTP and dTTP revealed Hoogsteen-like base pairing mechanisms. Two hydrogen bonds were observed in the N-2,3-epsilon G: dCTP base pair, whereas only one appears to be present in the case of the N-2,3-epsilon G: dTTP pair. Base pairing mechanisms derived from the crystal structures explain the slightly favored dCTP insertion for pol iota in steady-state kinetic analysis. Taken together, these results provide a basis for the mutagenic potential of N-2,3-epsilon G.
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