Journal
JOURNAL OF BIOLOGICAL CHEMISTRY
Volume 287, Issue 40, Pages 33826-33835Publisher
AMER SOC BIOCHEMISTRY MOLECULAR BIOLOGY INC
DOI: 10.1074/jbc.M112.380113
Keywords
-
Categories
Funding
- amfAR [107768-47-RGNT]
- Yongling Foundation
Ask authors/readers for more resources
The role of IL-7 in pre-T cell receptor (TCR) signaling during human T cell development is poorly understood. To study this, we engineered Molt3, a T cell progenitor T-acute lymphoblastic leukemia cell line, using lentiviral IL-7 receptor alpha (IL-7R alpha) to serve as a model system. IL-7 promoted pre-TCR activation in IL-7R alpha(hi) Molt3 as illustrated by CD25 up-regulation after anti-CD3 stimulation. Anti-CD3 treatment activated Akt and Erk1/2 signaling pathways as proven using specific inhibitors, and IL-7 further enhanced both signaling pathways. The close association of IL-7R alpha with CD3 zeta in the pre-TCR complex was illustrated through live imaging confocal fluorescence microscopy. These results demonstrate a direct and cooperative role of IL-7 in pre-TCR signaling.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available