Journal
JOURNAL OF BIOLOGICAL CHEMISTRY
Volume 286, Issue 20, Pages 17536-17542Publisher
AMER SOC BIOCHEMISTRY MOLECULAR BIOLOGY INC
DOI: 10.1074/jbc.M111.225532
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Funding
- Ministry of Education, Culture, Sports, Science, and Technology of Japan [19300138, 21300145]
- Ministry of Health, Labor, and Welfare of Japan [H20-007]
- National Center for Geriatrics and Gerontology, Japan [21-11]
- National Institutes of Health [R01AG027924, R01AG035355]
- Alzheimer Association
- Grants-in-Aid for Scientific Research [21300145, 19300138] Funding Source: KAKEN
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Apolipoprotein E (apoE) is a major apolipoprotein in the brain. The epsilon 4 allele of apoE is a major risk factor for Alzheimer disease, and apoE deficiency in mice leads to blood-brain barrier (BBB) leakage. However, the effect of apoE isoforms on BBB properties are as yet unknown. Here, using an in vitro BBB model consisting of brain endothelial cells and pericytes prepared from wild-type (WT) mice, and primary astrocytes prepared from human apoE3- and apoE4-knock- in mice, we show that the barrier function of tight junctions (TJs) was impaired when the BBB was reconstituted with primary astrocytes from apoE4-knock- in mice (apoE4-BBB model). The phosphorylation of occludin at Thr residues and the activation of protein kinase C (PKC)eta in mBECs were attenuated in the apoE4-BBB model compared with those in the apoE3- BBB model. The differential effects of apoE isoforms on the activation of PKC eta, the phosphorylation of occludin at Thr residues, and TJ integrity were abolished following the treatment with an anti-low density lipoprotein receptor-related protein 1 (LRP1) antibody or a LRP1 antagonist receptor-associated protein. Consistent with the results of in vitro studies, BBB permeability was higher in apoE4-knock- in mice than in apoE3- knock-in mice. Our studies provide evidence that TJ integrity in BBB is regulated by apoE in an isoform-dependent manner.
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