Journal
JOURNAL OF BIOLOGICAL CHEMISTRY
Volume 286, Issue 46, Pages 39904-39913Publisher
AMER SOC BIOCHEMISTRY MOLECULAR BIOLOGY INC
DOI: 10.1074/jbc.M111.267583
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- Wellcome Trust [RG50995]
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Alzheimer disease is characterized by neuronal loss and brain plaques of extracellular amyloid beta (A beta), but the means by which A beta may induce neuronal loss is not entirely clear. Although high concentrations of A beta (mu M) can induce direct toxicity to neurons, we find that low concentration (nM) induce neuronal loss through a microglia-mediated mechanism. In mixed neuronal-glial cultures from rat cerebellum, 250 nM A beta 1-42 (added as monomers, oligomers or fibers) induced about 30% loss of neurons between 2 and 3 days. This neuronal loss occurred without any increase in neuronal apoptosis or necrosis, and no neuronal loss occurred with A beta 42-1. A beta greatly increased the phagocytic capacity of microglia and induced phosphatidylserine exposure (an eat-me signal) on neuronal processes. Blocking exposed phosphatidylserine by adding annexin V or an antibody to phosphatidylserine or inhibiting microglial phagocytosis by adding either cytochalasin D (to block actin polymerization) or cyclo( RGDfV) (to block vitronectin receptors) significantly prevented neuronal loss. Loss of neuronal synapses occurred in parallel with loss of cell bodies and was also prevented by blocking phagocytosis. Inhibition of phagocytosis prevented neuronal loss with no increase in neuronal death, even after 7 days, suggesting that microglial phagocytosis was the primary cause of neuronal death induced by nanomolar A beta
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