Oxygen Supply from the Bird's Eye Perspective GLOBIN E IS A RESPIRATORY PROTEIN IN THE CHICKEN RETINA

The visual process in the vertebrate eye requires high amounts of metabolic energy and thus oxygen. Oxygen supply of the avian retina is a challenging task because birds have large eyes, thick retinae, and high metabolic rates but neither deep retinal nor superficial capillaries. Respiratory proteins such as myoglobin may enhance oxygen supply to certain tissues, and thus the mammalian retina harbors high amounts of neuroglobin. Globin E (GbE) was recently identified as an eye-specific globin of chicken (Gallus gallus). Orthologous GbE genes were found in zebra finch and turkey genomes but appear to be absent in non-avian vertebrate classes. Analyses of globin phylogeny and gene synteny showed an ancient origin of GbE but did not help to assign it to any specific globin type. We show that the photoreceptor cells of the chicken retina have a high level ofGbE protein, which accumulates to similar to 10 mu M in the total eye. Quantitative real-time RT-PCR revealed an similar to 50,000-fold higher level of GbE mRNA in the eye than in the brain. Spectroscopic analysis and ligand binding kinetics of recombinant chicken GbE reveal a penta-coordinated globin with an oxygen affinity of P-50 = 5.8 torrs at 25 degrees C and 15 torrs at 41 degrees C. Together these data suggest that GbE helps to sustain oxygen supply to the avian retina.