Journal
JOURNAL OF BIOLOGICAL CHEMISTRY
Volume 285, Issue 15, Pages 11681-11691Publisher
AMER SOC BIOCHEMISTRY MOLECULAR BIOLOGY INC
DOI: 10.1074/jbc.M109.096107
Keywords
-
Categories
Funding
- Ministry of Science, Education, Culture and Technology of Japan
- The Uehara Memorial Foundation
- ONO Medical Research Foundation
- Takeda Science Foundation
- Suzuken Memorial Foundation
- Japan Heart Foundation
- Kanae Foundation for the Promotion of Medical Science
- Senri Life Science Foundation
- Japan Foundation for Applied Enzymology
- Okinaka Memorial Institute for Medical Research
- Grants-in-Aid for Scientific Research [21591123] Funding Source: KAKEN
Ask authors/readers for more resources
Sterol regulatory element-binding protein (SREBP)-1 is a key transcription factor for the regulation of lipogenic enzyme genes in the liver. Polyunsaturated fatty acids (PUFA) selectively suppress hepatic SREBP-1, but molecular mechanisms remain largely unknown. To gain insight into this regulation, we established in vivo reporter assays to assess the activities of Srebf1c transcription and proteolytic processing. Using these in vivo reporter assays, we showed that the primary mechanism for PUFA suppression of SREBP-1 is at the proteolytic processing level and that this suppression in turn decreases the mRNA transcription through lowering SREBP-1 binding to the SREBP-binding element on the promoter (autoloop regulatory circuit), although liver X receptor, an activator for Srebf1c transcription, is not involved in this regulation by PUFA. The mechanisms for PUFA suppression of SREBP-1 confirm that the autoloop regulation for transcription is crucial for the nutritional regulation of triglyceride synthesis.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available