Journal
NEOTROPICAL ICHTHYOLOGY
Volume 13, Issue 1, Pages 157-164Publisher
SOC BRASILEIRA ICTIOLOGIA
DOI: 10.1590/1982-0224-20140066
Keywords
CASA; Characiformes; cryopreservation; Ovaprim (TM); sperm motility
Categories
Funding
- Brazilian fostering agency CNPq [PQ 302434/2011-9, 554950/2009-0, 142816/2009-4, 471393/2011-8]
- Brazilian fostering agency ANEEL PD Furnas [017965]
- Brazilian fostering agency FAPEMIG [PPM 00038-13, BPD 00167-12]
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The effects of reduced doses of Ovaprim (TM) (GnRHa + domperidone) on sperm release of Brycon orbignyanus and Prochilodus lineatus were evaluated. Furthermore, sperm quality was compared among fresh, equilibrated and postthaw samples. Males received a single and reduced dose of Ovaprim (TM) (0.125 or 0.25 ml/kg); control males received pituitary extract (cPE; 3 mg/kg). Fresh sperm was evaluated for volume, concentration, seminal plasma osmolality and seminal plasma pH. Then sperm was diluted in a freezing medium, equilibrated for 15-20 min and frozen in nitrogen vapor vessel (dry-shipper). Sperm motility was analyzed during 60 s post-activation in fresh, equilibrated and post-thaw samples. Sperm quality of males treated with Ovaprim (TM) (both doses) were not different from that of cPE-treated males, thus these data were pooled. In B. orbignyanus, motility was higher in fresh (99%) than in equilibrated sperm (81%); post-thaw motility dropped to 42%. In P. lineatus, motility was similar in fresh (99%) and equilibrated sperm (92%); post-thaw motility was 73%. Motility decreased as a function of time post-activation, and this decrease was significant after 60 s in fresh and equilibrated sperm, and as soon as 30 s in post-thaw sperm, in both species. Ovaprim (TM) at 1/4 of the recommended dose can successfully replace cPE.
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