Journal
JOURNAL OF BIOLOGICAL CHEMISTRY
Volume 285, Issue 44, Pages 33632-33641Publisher
AMER SOC BIOCHEMISTRY MOLECULAR BIOLOGY INC
DOI: 10.1074/jbc.M110.165761
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Funding
- National Institutes of Health [DK48873, DK56626, DK54320, HL59407]
- Grant Training Fellowship in Nutrition [T32 DK007647]
- Grant Training Fellowship in Translational Science [TL 1RR024158]
- American Diabetes Association
- Ara Parseghian Medical Research Foundation
- Bristol Myers Squibb
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Endoplasmic reticulum (ER) membrane cholesterol is maintained at an optimal concentration of similar to 5 mol % by the net impact of sterol synthesis, modification, and export. Arv1p was first identified in the yeast Saccharomyces cerevisiae as a key component of this homeostasis due to its probable role in intracellular sterol transport. Mammalian ARV1, which can fully complement the yeast lesion, encodes a ubiquitously expressed, resident ER protein. Repeated dosing of specific antisense oligonucleotides to ARV1 produced a marked reduction of ARV1 transcripts in liver, adipose, and to a lesser extent, intestine. This resulted in marked hypercholesterolemia, elevated serum bile acids, and activation of the hepatic farnesoid X receptor (FXR) regulatory pathway. Knockdown of ARV1 in murine liver and HepG2 cells was associated with accumulation of cholesterol in the ER at the expense of the plasma membrane and suppression of sterol regulatory element-binding proteins and their targets. These studies indicate a critical role of mammalian Arv1p in sterol movement from the ER and in the ensuing regulation of hepatic cholesterol and bile acid metabolism.
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