Journal
JOURNAL OF BIOLOGICAL CHEMISTRY
Volume 284, Issue 40, Pages 27281-27289Publisher
ELSEVIER
DOI: 10.1074/jbc.M109.022509
Keywords
-
Categories
Funding
- Cardiff University Link Chair Scheme
- Biotechnology and Biological Sciences Research Council (BBSRC) UK [BB/H001085/1]
- Medical Research Council [G0501963] Funding Source: researchfish
- MRC [G0501963] Funding Source: UKRI
Ask authors/readers for more resources
CD8(+) T-cells specific for MART-1-(26-35), a dominant melanoma epitope restricted by human leukocyte antigen (HLA)A* 0201, are exceptionally common in the naive T-cell repertoire. Remarkably, the TRAV12-2 gene is used to encode the T-cell receptor alpha(TCR alpha) chain in > 87% of these T-cells. Here, the molecular basis for this genetic bias is revealed from the structural and thermodynamic properties of an archetypal TRAV12-2-encoded TCR complexed to the clinically relevant heteroclitic peptide, ELAGIGILTV, bound to HLA-A*0201 (A2ELA). Unusually, the TRAV12-2 germ line-encoded regions of the TCR dominate the major atomic contacts with the peptide at the TCR/A2-ELA interface. This innate pattern of antigen recognition probably explains the unique characteristics and extraordinary frequencies of CD8(+) T-cell responses to this epitope.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available