Journal
JOURNAL OF BIOLOGICAL CHEMISTRY
Volume 284, Issue 41, Pages 27974-27979Publisher
AMER SOC BIOCHEMISTRY MOLECULAR BIOLOGY INC
DOI: 10.1074/jbc.M109.037929
Keywords
-
Categories
Funding
- CNRS
- European Union 6th Framework Programme TRANS-REG [LSHG-CT-2004-502950]
- SIROCCO [LSHG-CT-2006-037900]
Ask authors/readers for more resources
By combining biochemical purification and mass spectrometry, we identified proteins associated with human heterochromatin protein 1 alpha (HP1 alpha) both in the nucleoplasm and in chromatin. Some of these are RNA-binding proteins, and among them is the protein heterogeneous nuclear ribonucleoprotein U (hnRNP U)/SAF-A, which is linked to chromatin organization and transcriptional regulation. Here, we demonstrate that hnRNP U is a bona fide HP1 alpha-interacting molecule. More importantly, hnRNP U depletion reduces HP1 alpha-dependent gene silencing and disturbs HP1 alpha subcellular localization. Thus, our data demonstrate that hnRNP U is involved in HP1 alpha function, shedding new light on the mode of action of HP1 alpha and on the function of hnRNP U.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available