Journal
JOURNAL OF BIOLOGICAL CHEMISTRY
Volume 284, Issue 25, Pages 16914-16921Publisher
AMER SOC BIOCHEMISTRY MOLECULAR BIOLOGY INC
DOI: 10.1074/jbc.M109.003681
Keywords
-
Categories
Funding
- Hungarian Scientific Research Fund [NK-072661, NF-68563, M-045341, T-034606]
- Agency for Research Fund Management and Research Exploitation, Hungary [NKFP1-010/2005]
- Hungarian Ministry of Public Health [ETT 447/2006, 460/2006]
- EC [LSHM-CT-2004-503474]
Ask authors/readers for more resources
Intracellular signaling systems of G protein-coupled receptors are well established, but their role in paracrine regulation of adjacent cells is generally considered as a tissue-specific mechanism. We have shown previously that AT(1) receptor (AT(1)R) stimulation leads to diacylglycerol lipase-mediated transactivation of co-expressed CB(1)Rs in Chinese hamster ovary cells. In the present study we detected a paracrine effect of the endocannabinoid release from Chinese hamster ovary, COS7, and HEK293 cells during the stimulation of AT(1) angiotensin receptors by determining CB1 cannabinoid receptor activity with bioluminescence resonance energy transfer-based sensors of G protein activation expressed in separate cells. The angiotensin II-induced, paracrine activation of CB1 receptors was visualized by detecting translocation of green fluorescent protein-tagged beta-arrestin2. Mass spectrometry analyses have demonstrated angiotensin II-induced stimulation of 2-arachidonoylglycerol production, whereas no increase of anandamide levels was observed. Stimulation of G(q/11)-coupled M-1, M-3, M-5 muscarinic, V1 vasopressin, alpha(1a) adrenergic, B2 bradykinin receptors, but not G(i/o)-coupled M-2 and M-4 muscarinic receptors, also led to paracrine transactivation of CB1 receptors. These data suggest that, in addition to their retrograde neurotransmitter role, endocannabinoids have much broader paracrine mediator functions during activation of G(q/11)-coupled receptors.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available