Journal
JOURNAL OF BIOLOGICAL CHEMISTRY
Volume 283, Issue 29, Pages 20443-20453Publisher
AMER SOC BIOCHEMISTRY MOLECULAR BIOLOGY INC
DOI: 10.1074/jbc.M802866200
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Funding
- Medical Research Council [G0701446] Funding Source: Medline
- NIGMS NIH HHS [R01 GM028140, GM 28140] Funding Source: Medline
- Wellcome Trust Funding Source: Medline
- MRC [G0701446] Funding Source: UKRI
- Medical Research Council [G0701446] Funding Source: researchfish
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The Saccharomyces cerevisiae DGK1 gene encodes a diacylglycerol kinase enzyme that catalyzes the formation of phosphatidate from diacylglycerol. Unlike the diacylglycerol kinases from bacteria, plants, and animals, the yeast enzyme utilizes CTP, instead of ATP, as the phosphate donor in the reaction. Dgk1p contains a CTP transferase domain that is present in the SEC59-encoded dolichol kinase and CDS1-encoded CDP-diacylglycerol synthase enzymes. Deletion analysis showed that the CTP transferase domain was sufficient for diacylglycerol kinase activity. Point mutations (R76A, K77A, D177A, and G184A) of conserved residues within the CTP transferase domain caused a loss of diacylglycerol kinase activity. Analysis of DGK1 alleles showed that the in vivo functions of Dgk1p were specifically due to its diacylglycerol kinase activity. The DGK1-encoded enzyme had a pH optimum at 7.0-7.5, required Ca2(+) or Mg2(+) ions for activity, was potently inhibited by N-ethylmaleimide, and was labile at temperatures above 40 C. The enzyme exhibited positive cooperative (Hill number = 2.5) kinetics with respect to diacylglycerol (apparent K-m = 6.5 mol %) and saturation kinetics with respect to CTP (apparent K-m = 0.3mM). dCTP was both a substrate (apparent K-m = 0.4 mM) and competitive inhibitor (apparent K-i = 0.4 mM) of the enzyme. Diacylglycerol kinase activity was stimulated by major membrane phospholipids and was inhibited by CDP-diacylglycerol and sphingoid bases.
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