Journal
JOURNAL OF BIOLOGICAL CHEMISTRY
Volume 284, Issue 6, Pages 3563-3576Publisher
AMER SOC BIOCHEMISTRY MOLECULAR BIOLOGY INC
DOI: 10.1074/jbc.M807778200
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Funding
- National Institutes of Health Grants [R01 ES010375, F32 CA119776, P01 ES05355, P30 ES000267]
- U. S. Department of Energy, Basic Energy Sciences, Office of Science [W-31-109-Eng-38]
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In contrast to replicative DNA polymerases, Sulfolobus solfataricus Dpo4 showed a limited decrease in catalytic efficiency (k(cat)/K-m) for insertion of dCTP opposite a series of N-2-alkylguanine templates of increasing size from (methyl (Me) to (9-anthracenyl)-Me (Anth)). Fidelity was maintained with increasing size up to (2-naphthyl)-Me (Naph). The catalytic efficiency increased slightly going from the N-2-NaphG to the N-2-AnthG substrate, at the cost of fidelity. Pre-steady-state kinetic bursts were observed for dCTP incorporation throughout the series (N-2-MeG to N-2-AnthG), with a decrease in the burst amplitude and k(pol), the rate of single-turnover incorporation. The pre-steady-state kinetic courses with G and all of the six N-2-alkyl G adducts could be fit to a general DNA polymerase scheme to which was added an inactive complex in equilibrium with the active ternary Dpo4.DNA.dNTP complex, and only the rates of equilibrium with the inactive complex and phosphodiester bond formation were altered. Two crystal structures of Dpo4 with a template N-2-NaphG (in a post-insertion register opposite a 3'-terminal C in the primer) were solved. One showed N-2-NaphG in a syn conformation, with the naphthyl group located between the template and the Dpo4 little finger domain. The Hoogsteen face was within hydrogen bonding distance of the N4 atoms of the cytosine opposite N-2-NaphG and the cytosine at the -2 position. The second structure showed N-2-Naph G in an anti conformation with the primer terminus largely disordered. Collectively these results explain the versatility of Dpo4 in bypassing bulky G lesions.
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