Journal
JOURNAL OF BIOLOGICAL CHEMISTRY
Volume 284, Issue 5, Pages 3012-3020Publisher
AMER SOC BIOCHEMISTRY MOLECULAR BIOLOGY INC
DOI: 10.1074/jbc.M806163200
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- Max-Planck-Gesellschaft
- American Cancer Society [RSG-9508506-DDC, 1-FY07-527]
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Body pattern formation during early embryogenesis of Drosophila melanogaster relies on a zygotic cascade of spatially restricted transcription factor activities. The gap gene Kruppel ranks at the top level of this cascade. It encodes a C2H2 zinc finger protein that interacts directly with cis-acting stripe enhancer elements of pair rule genes, such as even skipped and hairy, at the next level of the gene hierarchy. Kruppel mediates their transcriptional repression by direct association with the corepressor Drosophila C terminus-binding protein (dCtBP). However, for some Kruppel target genes, deletion of the dCtBP-binding sites does not abolish repression, implying a dCtBP-independent mode of repression. We identified Kruppel-binding proteins by mass spectrometry and found that SAP18 can both associate with Kruppel and support Kruppel-dependent repression. Genetic interaction studies combined with pharmacological and biochemical approaches suggest a site-specific mechanism of Kruppel-dependent gene silencing. The results suggest that Kruppel tethers the SAP18 bound histone deacetylase complex 1 at distinct enhancer elements, which causes repression via histone H3 deacetylation.
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