Journal
JOURNAL OF BIOLOGICAL CHEMISTRY
Volume 283, Issue 43, Pages 29144-29155Publisher
AMER SOC BIOCHEMISTRY MOLECULAR BIOLOGY INC
DOI: 10.1074/jbc.M804596200
Keywords
-
Categories
Funding
- Ministry of Education, Culture, Sports, Science and Technology of Japan
Ask authors/readers for more resources
Tyr(122)-hydrophobic cluster (Y122-HC) is an interaction network formed by the top part of the second transmembrane helix and the cytoplasmic actuator and phosphorylation domains of sarcoplasmic reticulum Ca2+-ATPase. We have previously found that Y122-HC plays critical roles in the processing of ADP-insensitive phosphoenzyme (E2P) after its formation by the isomerization from ADP-sensitive phosphoenzyme (E1PCa(2)) (Wang, G., Yamasaki, K., Daiho, T., and Suzuki, H. (2005) J. Biol. Chem. 280, 26508-26516). Here, we further explored kinetic properties of the alanine-substitution mutants of Y122-HC to examine roles of Y122-HC for Ca2+ release process in E2P. In the steady state, the amount of E2P decreased so that of E1PCa(2) increased with increasing lumenal Ca2+ concentration in the mutants with K-0.5 110-320 mu M at pH 7.3. These lumenal Ca2+ affinities in E2P agreed with those estimated from the forward and lumenal Ca2+-induced reverse kinetics of the E1PCa(2)-E2P isomerization. K-0.5 of the wild type in the kinetics was estimated to be 1.5 mM. Thus, E2P of the mutants possesses significantly higher affinities for lumenal Ca2+ than that of the wild type. The kinetics further indicated that the rates of lumenal Ca2+ access and binding to the transport sites of E2P were substantially slowed by the mutations. Therefore, the proper formation of Y122-HC and resulting compactly organized structure are critical for both decreasing Ca2+ affinity and opening the lumenal gate, thus for Ca2+ release from E2PCa(2). Interestingly, when K+ was omitted from the medium of the wild type, the properties of the wild type became similar to those of Y122-HC mutants. K+ binding likely functions via producing the compactly organized structure, in this sense, similarly to Y122-HC.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available