Journal
JOURNAL OF BIOENERGETICS AND BIOMEMBRANES
Volume 45, Issue 1-2, Pages 47-57Publisher
SPRINGER/PLENUM PUBLISHERS
DOI: 10.1007/s10863-012-9481-9
Keywords
Long-chain 3-hydroxy fatty acids; Mitochondria; Heart bioenergetics; Oxidative phosphorylation; Permeability transition
Categories
Funding
- CNPq
- PRONEX II
- FAPERGS
- FAPESP
- PROPESQ/UFRGS
- FINEP Rede Instituto Brasileiro de Neurociencia (IBN-Net) [01.06.0842-00]
- Instituto Nacional de Ciencia e Tecnologia, Excitotoxicidade e Neuroprotecao (INCT-EN)
Ask authors/readers for more resources
Cardiomyopathy is a common clinical feature of some inherited disorders of mitochondrial fatty acid beta-oxidation including mitochondrial trifunctional protein (MTP) and isolated long-chain 3-hydroxyacyl-CoA dehydrogenase (LCHAD) deficiencies. Since individuals affected by these disorders present tissue accumulation of various fatty acids, including long-chain 3-hydroxy fatty acids, in the present study we investigated the effect of 3-hydroxydecanoic (3 HDCA), 3-hydroxydodecanoic (3 HDDA), 3-hydroxytetradecanoic (3 HTA) and 3-hydroxypalmitic (3 HPA) acids on mitochondrial oxidative metabolism, estimated by oximetry, NAD(P)H content, hydrogen peroxide production, membrane potential (Delta I) and swelling in rat heart mitochondrial preparations. We observed that 3 HTA and 3 HPA increased resting respiration and diminished the respiratory control and ADP/O ratios using glutamate/malate or succinate as substrates. Furthermore, 3 HDDA, 3 HTA and 3 HPA decreased Delta I, the matrix NAD(P)H pool and hydrogen peroxide production. These data indicate that these fatty acids behave as uncouplers of oxidative phosphorylation. We also verified that 3 HTA-induced uncoupling-effect was not mediated by the adenine nucleotide translocator and that this fatty acid induced the mitochondrial permeability transition pore opening in calcium-loaded organelles since cyclosporin A prevented the reduction of mitochondrial Delta I and swelling provoked by 3 HTA. The present data indicate that major 3-hydroxylated fatty acids accumulating in MTP and LCHAD deficiencies behave as strong uncouplers of oxidative phosphorylation potentially impairing heart energy homeostasis.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available