Journal
JOURNAL OF BIOENERGETICS AND BIOMEMBRANES
Volume 40, Issue 6, Pages 599-606Publisher
SPRINGER/PLENUM PUBLISHERS
DOI: 10.1007/s10863-008-9186-2
Keywords
Post-conditioning; Mitochondria; Permeability transition pore; Ischemic-reperfused heart; CSA, cyclosporin A
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Funding
- National Council of Science and Technology (CONACyT), Mexico [80791-M]
- CONACyT
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The pathways activated by post-conditioning may converge on the mitochondria, in particular on the mitochondrial permeability transition pore. We sought to characterize the inhibition status of the mitochondrial permeability transition early after the post-conditioning maneuver and before long reperfusion was established. We observed that post-conditioning maneuvers applied to isolated rat hearts, after a prolonged ischemia and before reperfusion, promoted cardiac mechanical function recovery and maintained mitochondrial integrity. These effects were evaluated by mitochondrial swelling, calcium transport, and NAD(+) content measurements; the improvements were established before restoring a long lasting reperfusion period. Mitochondrial integrity was associated with a diminution in oxidative stress, since carbonylation of proteins was prevented and aconitase activity was preserved in the post-conditioned hearts, implying that ROS might mediate mitochondrial dysfunction and mPTP opening. In addition, we found that cytochrome release was significantly abolished in the post-conditioned heart, in contrast with conventionally reperfused hearts.
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