Journal
JOURNAL OF BIOCHEMISTRY
Volume 155, Issue 6, Pages 361-373Publisher
OXFORD UNIV PRESS
DOI: 10.1093/jb/mvu012
Keywords
cytotoxicity; inhibition; insulin fibrillation; nucleation; paclitaxel
Categories
Funding
- University of Tehran
- Center of Excellence in Biothermodynamics (CEBiotherm)
- Iran National Science Foundation (INSF)
- Iran National Elites Foundation (INEF)
- UNESCO Chair on Interdisciplinary Research in Diabetes at University of Tehran
Ask authors/readers for more resources
Alzheimer, a neurodegenerative disease, and a large variety of pathologic conditions are associated with a form of protein aggregation known as amyloid fibrils. Since fibrils and prefibrillar intermediates are cytotoxic, numerous attempts have been made to inhibit fibrillation process as a therapeutic strategy. Peptides, surfactants and aromatic small molecules have been used as fibrillation inhibitors. Here we studied the effects of paclitaxel, a polyphenol with a high tendency for interaction with proteins, on fibrillation of insulin as a model protein. The effects of paclitaxel on insulin fibrillation were determined by Thioflavin T fluorescence, Congo red absorbance, circular dichroism and atomic force microscopy. These studies indicated that paclitaxel considerably hindered nucleation, and therefore, fibrillation of insulin in a dose-dependant manner. The isothermal titration calorimetry studies showed that the interaction between paclitaxel and insulin was spontaneous. In addition, the van der Waal's interactions and hydrogen bonds were prominent forces contributing to this interaction. Computational results using molecular dynamic simulations and docking studies revealed that paclitaxel diminished the polarity of insulin dimer and electrostatic interactions by increasing the hydrophobicity of its dimer state. Furthermore, paclitaxel reduced disrupting effects of insulin fibrils on PC12 cell's neurite outgrowth and complexity, and enhanced their survival.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available