Journal
JOURNAL OF BIOCHEMICAL AND BIOPHYSICAL METHODS
Volume 70, Issue 6, Pages 1240-1246Publisher
ELSEVIER
DOI: 10.1016/j.jprot.2007.11.005
Keywords
chitosan; microspheres; nanoparticles; beta-galactosidase; immobilization
Ask authors/readers for more resources
Macro-, micro- and nanosized chitosan particles suitable as immobilization carriers were prepared by precipitation, emulsion cross-linking and ionic gelation methods, respectively. Effects of particle preparation parameters on particle size were investigated. Activities of beta-galactosidase covalently attached to differently sized particles have been evaluated and compared. The highest activity was shown by the biocatalyst immobilized on nanoparticles obtained by means of the ionotropic gelation method with sodium sulphate as gelation agent. beta-Galactosidase fixed on macro- and microspheres exhibited excellent storage stability in aqueous solution, with no more than 5% loss of activity after 3 weeks storage at 4 degrees C and pH 7.0. (c) 2007 Elsevier B.V. All rights reserved.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available