Journal
JOURNAL OF BIOACTIVE AND COMPATIBLE POLYMERS
Volume 29, Issue 2, Pages 137-151Publisher
SAGE PUBLICATIONS LTD
DOI: 10.1177/0883911514521919
Keywords
scaffold; biocompatibility; biodegradation; subcutaneous implantation; Tissue engineering; chitosan
Funding
- Portuguese Fundacao para a Ciencia e a Tecnologia (FCT) [SFRH/24735/2005]
- European Network of Excellence EXPERTISSUES [NMP3-CT-2004-500283]
- FCT [PTDC/SAU-ENB/115179/2009]
- Fundação para a Ciência e a Tecnologia [PTDC/SAU-ENB/115179/2009] Funding Source: FCT
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In tissue engineering, the evaluation of the host response to the biomaterial implantation must be assessed to determine the extent of the inflammatory reaction. We studied the degradation of poly(butylene succinate) and chitosan in vitro using lipase and lysozyme enzymes, respectively. The subcutaneous implantation of the scaffolds was performed to assess tissue response. The type of inflammatory cells present in the surrounding tissue, as well as within the scaffold, was determined histologically and by immunohistochemistry. In the presence of lipase or lysozyme, the water uptake of the scaffolds increased. Based on the weight loss data and scanning electron microscopy analysis, the lysozyme combined with lipase had a notable effect on the in vitro degradation of the scaffolds. The in vivo implantation showed a normal inflammatory response, with presence of neutrophils, in a first stage, and macrophages, lymphocytes, and giant cells in a later stage. Vascularization in the surrounding tissue and within the implant increased with time. Moreover, the collagen deposition increased with time inside the implant. In vivo, the scaffolds maintained the structural integrity. The degradation in vitro was faster and greater compared to that observed in vivo within the same time periods.
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