Journal
JOURNAL OF BIOACTIVE AND COMPATIBLE POLYMERS
Volume 27, Issue 2, Pages 174-182Publisher
SAGE PUBLICATIONS LTD
DOI: 10.1177/0883911511434961
Keywords
algiantes; mesenchymal stem cells; viability; guluronic acid; mannuronic acid
Funding
- Nova Southeastern University
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In this study, the effect of pharmaceutical-grade alginates on the cell viability of human mesenchymal stem cells derived from umbilical cord was examined and their use in tissue engineering applications was evaluated. The effects of the ratio of the copolymer building blocks (guluronic and mannuronic acids) and their interactions with divalent calcium, the purity of alginates (proteins and polyphenol content), and gelation factors (calcium concentration and sol content) were examined. The high guluronic acid content in the alginates improved the viability of the human mesenchymal stem cells derived from umbilical cord and supported cell growth significantly. It was confirmed that the sol fraction of alginate reduced cell viability. Cells in the presence of alginate beads cross-linked with 50 and 100 mM calcium chloride showed maximum viability; the protein and polyphenol content of the alginates did not affect the viability of the human mesenchymal stem cells derived from umbilical cord, while the monomer ratio did have an obvious effect.
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