Journal
JOURNAL OF BIOACTIVE AND COMPATIBLE POLYMERS
Volume 26, Issue 5, Pages 452-463Publisher
SAGE PUBLICATIONS LTD
DOI: 10.1177/0883911511419970
Keywords
biopolyester; poly(3-hydroxybutyrate); thermally induced phase separation; lipase; glucosamine; gelatin; carbodiimide coupling
Funding
- Swedish International Development Cooperation Agency (Sida)
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Scaffolds made from poly(3-hydroxybutyrate) were prepared by thermally induced phase separation from solutions in dioxane at temperatures above (23 degrees C) or below the freezing point of the solvent. At 23 degrees C, gelation occured with nano-fibrous network formation. The scaffolds prepared at -12 degrees C and -25 degrees C exhibited a highly porous morphology, with pores in the range of 3-25 mu m, caused by freezing of dioxane. The macroporous scaffolds, prepared at -12 degrees C, were treated with lipase to generate functional groups to which gelatin and glucosamine, respectively, were chemically coupled. The modified scaffolds had lower molecular weight, higher water content, lower melting temperature, and enthalpy. Cultivation of human embryonic fibroblasts on the macroporous scaffolds confirmed that the cells proliferated and adhered to the materials.
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