Journal
JOURNAL OF BIOACTIVE AND COMPATIBLE POLYMERS
Volume 26, Issue 3, Pages 242-256Publisher
SAGE PUBLICATIONS LTD
DOI: 10.1177/0883911511406327
Keywords
micropattern; gradient pattern; cell density; tissue engineering; stem cell; cell function; poly(vinyl alcohol)
Funding
- Ministry of Education, Culture, Sports, Science and Technology, Japan
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A micropatterned surface with different area ratios of cell-adhesive to nonadhesive surfaces was prepared by micropatterning poly(vinyl alcohol) on a polystyrene plate using photolithography. A gradient pattern of mesenchymal stem cells of different cell densities was generated by culturing the cells on a micropatterned surface. The effects of the cell density gradient on cell functions such as proliferation and differentiation were investigated. Cells seeded at a low density proliferated faster than cells seeded at a high density. Although mesenchymal stem cells seeded at both low and high densities showed osteogenic differentiation, the higher cell seeding density could initiate osteogenic differentiation at a faster rate than the low cell density. And high cell density was required to induce chondrogenic differentiation.
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