Journal
JOURNAL OF BIOACTIVE AND COMPATIBLE POLYMERS
Volume 25, Issue 1, Pages 40-57Publisher
SAGE PUBLICATIONS LTD
DOI: 10.1177/0883911509353680
Keywords
electrospinning; chitosan; enzyme immobilization; acetylcholinesterase; polyacrylonitrile; enhanced enzyme activity; enhanced enzyme stability; enhanced enzyme durability
Funding
- National Science Fund [TC-Ch-1605]
- Operational Program 'Human Resources Development' [BG051PO001/07/3.3-02/51]
Ask authors/readers for more resources
Nanofibrous polyacrylonitrile membranes (PANNFM) were obtained by electrospinning and then prepared for immobilizing acetylcholinesterase (AChE). Initially, the chemical modification of PANNFM with ethylenediamine produced reactive groups to overcome their inertness and hydrophobicity. The natural polymer, chitosan, was then tethered on the nanofibrous membranes to improve their biocompatibility. Scanning electron microscopy (SEM) and cross-section SEM were used to determine morphological and porosity changes of the membranes. The immobilized AChE had greater relative activity as well as thermal and storage stability compared to the free enzyme. The bound AChE showed excellent reusability. Chitosan-modified PANNFM was shown to be a suitable strategy for facile immobilization of AChE to produce a promising system that effectively supports biocatalysts.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available