Journal
JOURNAL OF BASIC MICROBIOLOGY
Volume 54, Issue 8, Pages 835-842Publisher
WILEY-BLACKWELL
DOI: 10.1002/jobm.201200534
Keywords
Filobasidium capsuligenum; Pectinolytic yeasts; Wine; San Rafael (Mendoza / Argentina) viticulture region
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Funding
- UNCuyo [06/L096]
- I+D (UNCuyo)
- ANPCyT (PICT) [0847]
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In this study indigenous yeasts associated with wineries, grapes and Malbec fermented must from San Rafael viticulture region (Argentina) were isolated to select pectinolytic strains for their potential use in enology. Pectinolytic yeasts were identified by physiological and molecular methods. Among 78 isolates, only nine were able to produce extracellular pectinases. Six isolated from berry surface were identified as Aureobasidium pullulans and the remaining isolates, recovered from wineries, belonged to Saccharomyces cerevisiae and Filobasidium capsuligenum species. Pectinase production was evaluated under vinification-related conditions: pH 3.5, 12 and 28 degrees C. A. pullulans U-12 produced the highest pectinolytic activity at low temperature (1.16 U ml(-1)), while F. capsuligenum strains showed good activity at 12 and 28 degrees C (0.77 and 1.15 U ml(-1), respectively) being this study the first report on the capacity of this species to produce pectinases. The pectinolytic activity of F. capsuligenum B-13 showed an optimum at pH 4.5 and two peaks at 20 and 50 degrees C. The enzyme half-life was 2 h at 40 degrees C and retained 65% of its activity at 40 degrees C after 1 h of incubation. This pectinolytic system displayed remarkable activity at pH and temperatures found in vinification, suggesting a potential candidate for applying to wine-making.
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