Journal
JOURNAL OF BASIC MICROBIOLOGY
Volume 54, Issue 2, Pages 133-141Publisher
WILEY
DOI: 10.1002/jobm.201200325
Keywords
Penicillium griseoroseum; Pectin lyase; Polygalacturonase; Secretion; Mycelial morphology
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Funding
- Brazilian Foundation for Research Support of Minas Gerais (FAPEMIG)
- Coordination for the Improvement of Higher Education Personnel (CAPES)
- National Council for Scientific and Technological Development (CNPq)
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A number of parameters, including culture medium pH, affect growth and enzyme production by microorganisms. In the present study, the production and secretion of pectin lyase (PL) and polygalacturonase (PG) by recombinant strains of Penicillium griseoroseum cultured in mineral-buffered media (MBM; initial pH 6.8) and mineral-unbuffered medium (MUM; initial pH 6.3) were evaluated. Under these culture conditions, no change in the transcriptional levels of plg1 and pgg2 was observed. However, the levels of secreted total protein ranged from 7.80 +/- 1.1 to 3.25 +/- 1.50 mu gml(-1) in MBM and MUM, respectively, and were evaluated by SDS-PAGE. PL and PG enzymatic activities decreased 6.4 and 3.6 times, respectively, when P. griseoroseum was cultivated under acidic pH conditions (MUM). Furthermore, differences were observed in the hypha and mycelium morphology. These findings suggest that acidic growing conditions affect PL and PG secretion, even though the transcription and translation processes are successful. The data obtained in this study will help to establish optimal culture conditions that increase production and secretion of recombinant proteins by filamentous fungi.
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