Journal
JOURNAL OF BASIC MICROBIOLOGY
Volume 54, Issue 2, Pages 89-96Publisher
WILEY
DOI: 10.1002/jobm.201200229
Keywords
Lectins; Basidiomycetes; Affinity chromatography; Glycoproteins
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Funding
- Comision Sectorial de Investigacion Cientifica (CSIC-UdelaR) [325]
- Program for Development of Basic Sciences (PEDECIBA-QUIMICA)
- Agencia Nacional de Investigacion e Innovacion (ANII), Uruguay
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Aqueous extracts of basidiomycete fungi were screened for the presence of lectins by hemagglutination (HA) assays with mouse, rabbit, and sheep red blood cells. From mycelia and/or fruiting bodies, 23 extracts were prepared; 15 extracts exhibited HA activity towards mouse erythrocytes, with specific activities ranging from 12 to 440 lectin units (LU)mg(-1) protein. In HA inhibition assays, 43 carbohydrates including mono-, di-, tri-, tetrasaccharides, glycoproteins, and polysaccharides were tested as haptens, to determine the saccharide-binding specificities of the lectins. A novel lectin with specificity towards N-acetyl-glucosamine was purified from mycelia of Punctularia atropurpurascens using affinity chromatography on chitosan-Sepharose. The lectin has a subunit molecular mass of 67kDa determined by SDS-PAGE and a pI of 5.0.
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