Indigo production by Pseudomonas sp J26, a marine naphthalene-degrading strain

A technique developed to determine naphthalene dioxygenase (NDO) activity was optimized and used to study the biotransformation of indole to indigo by Pseudomonas sp. J26 whole cells. The maximum production of indigo was achieved at 25 degrees C using 2.5 mM indole when J26 was grown in the complex medium JPP, while indole concentrations higher than 4 mM proved toxic for cells. The maximum rate of indigo production was 0.56 nmol min(-1) mg dry biomass(-1), obtaining 75.5 mu M of indigo after 8 h of incubation, while a maximal concentration (138.1 mu M) of indigo was obtained after 20 h.