Journal
JOURNAL OF BASIC MICROBIOLOGY
Volume 49, Issue 6, Pages 553-563Publisher
WILEY-BLACKWELL
DOI: 10.1002/jobm.200900089
Keywords
Trichosporon montevideense; Biosurfactant; Emulsifying
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Funding
- Conselho Nacional de Desenvolvimento Cienti fico e Tecnologico (CNPq)
- Fundacao do Amparo a Pesquisa do Estado de Minas Gerais (FAPEMIG)
- Comissao de Aperfeicoamento de Pessoal de Nivel Superior (CAPES)
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The yeast strain CLOA 72 isolated from the effluent of a dairy industry in Brazil and identified as Trichosporon montevideense, was able to grow and produce a glycolipid biosurfactant when cultured on a mineral medium (MM) with sunflower oil as the carbon source. Biosurfactant production was partially growth-associated and maximal emulsification activity was observed at 144 h of cultivation (78.92%). The biosurfactant purified by precipitation with ethanol showed 78.66% emulsifying activity when used in concentrations above 4.5 mg/ml and was able to reduce the surface tension of water to values below 44.9 mN/m. The critical micellar concentration (CMC) was found to be 2.2 mg/ml. The highest emulsifying activity (E-24) has been observed with vegetable oils, toluene, kerosene, isooctane, cyclohexane, hexane, diesel oil and hexadecane as compared to mineral oil and oleic acid. The biosurfactant also showed good stability during exposure to 100 degrees C for different periods of time (10 to 60 min), to high salinity (30% of NaCl, KCl and NaHCO3), and to a wide range of pH values (1-10). The biosurfactant purified by gel filtration chromatography is a glycolipid, with lipid portion containing 16.03%. (9Z)-octadec-9-enoic acid, 14.92% hexadecanoic acid, and 9.63% (E) octadec-9-enoic acid and the carbohydrate portion containing mannose (35.29%), xylose (41.99%), arabinose (17.47%), and glucose (5.25%).
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