Journal
JOURNAL OF BASIC MICROBIOLOGY
Volume 49, Issue 2, Pages 212-215Publisher
WILEY-V C H VERLAG GMBH
DOI: 10.1002/jobm.200800186
Keywords
Bioreactor; Fed-batch; Lipase; Yarrowia lipolytica
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Two different types of fed-batch fermentation were investigated to improve production yields of the Lip2 extracellular lipase in Y. lipolytica mutant-strain LgX64.81 grown in a 201 bioreactor. Compare to batch cultures, culture feeding with the complete medium led to a 2-fold increased lipase production (2016 +/- 76 U ml(-1)) whereas addition of a combination of glucose and olive oil led to a 3-fold increase. The high level of lipase production obtained on glucose media with Y. lipolytica LgX64.81 could be related to its phenotype i.e. a lower sensibility to glucose catabolite repression due to a modification in the level of HXK1 expression.
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