Journal
JOURNAL OF BASIC MICROBIOLOGY
Volume 48, Issue 4, Pages 315-318Publisher
WILEY
DOI: 10.1002/jobm.200800038
Keywords
chaperone oligonucleotide microarray; 16S-23S internal transcribed spacer; Kitasatospora
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Identification of members of the genus Kitasatospora from soil samples has been introduced to evaluate occurrence of potential natural compound producers in different habitats. The microarray hybridization usually involves PCR amplification of the target DNA. Since PCR might lead to biased amplification, a diagnostic Kitasatospora microarray technique was improved by a protocol lacking PCR amplification prior to hybridization. The described advanced hybridization method used chaperone oligonucleotides for direct co-hybridization with genomic DNA on an oligonuclotide microarray with optical readout.
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