Journal
JOURNAL OF BASIC MICROBIOLOGY
Volume 48, Issue 5, Pages 370-377Publisher
WILEY
DOI: 10.1002/jobm.200800036
Keywords
Olive knots; Auxin; Pseudomonas savastanoi; 16S-23S intergenic spacer
Categories
Funding
- Ministry of High Education, Scientific Research and Technology of the Tunisian Republic [MBA 2051206, CRTE 06]
Ask authors/readers for more resources
Forty three strains were isolated from knots induced by Pseudomonas savastanoi in different olive cultivars. All the selected bacteria were shown to produce variable amounts of the plant growth hormone indole-3-acetic acid (IAA). Amplification of the intergenic transcribed spacers (ITS) between 16S and 23S rDNA genes, allowed the clustering of the isolates into seven distinct groups. All isolates from ITS group 1 were positive to the Pseudomonas savastanoi pv. savastanoi specific iaaL gene as shown by PCR. Partial sequencing of 16S rDNA gene confirmed the identity of these isolates to Pseudomonas savastanoi strains and allowed to tentatively assign the other isolates from the remaining ITS groups to Pantoea oleae/agglomerans, Burkholderia cepacia, Pseudomonas putida, Stenotrophomonas maltophilia and Hafnia alvei. Identification of endophytic knot-derived isolates revealed association of various saprophytic and putative human pathogenic bacteria with P. savastanoi pv. savastanoi in knot environment of olive infected trees.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available