Journal
JOURNAL OF BACTERIOLOGY
Volume 193, Issue 7, Pages 1771-1776Publisher
AMER SOC MICROBIOLOGY
DOI: 10.1128/JB.01292-10
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Funding
- DEFRA [OZ0707]
- BBSRC [D017556, D017947]
- Wellcome Trust
- Biotechnology and Biological Sciences Research Council [BBS/E/I/00000715, BB/D017947/1] Funding Source: researchfish
- BBSRC [BB/D017947/1] Funding Source: UKRI
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Massively parallel sequencing of transposon-flanking regions assigned the genotype and fitness score to 91% of Escherichia coli O157:H7 mutants previously screened in cattle by signature-tagged mutagenesis (STM). The method obviates the limitations of STM and markedly extended the functional annotation of the prototype E. coli O157:H7 genome without further animal use.
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