Journal
JOURNAL OF BACTERIOLOGY
Volume 193, Issue 12, Pages 3100-3108Publisher
AMER SOC MICROBIOLOGY
DOI: 10.1128/JB.01460-10
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Funding
- BBSRC [BB/H006125/1]
- Biotechnology and Biological Sciences Research Council [BBS/E/J/00000015, BB/H006125/1] Funding Source: researchfish
- BBSRC [BB/H006125/1] Funding Source: UKRI
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We describe the identification and functional characterization of cdgB (SCO4281), a recently discovered target of BldD, a key regulator of morphological differentiation and antibiotic production in the mycelial bacteria of the genus Streptomyces. cdgB (cyclic dimeric GMP [c-di-GMP] B) encodes a GGDEF-containing protein that has diguanylate cyclase activity in vitro. Consistent with this enzymatic activity, heterologous expression of cdgB in Escherichia coli resulted in increased production of extracellular matrix in colonies and enhanced surface attachment of cells in standing liquid cultures. In Streptomyces coelicolor, both overexpression and deletion of cdgB inhibited aerial-mycelium formation, and overexpression also inhibited production of the antibiotic actinorhodin, implicating c-di-GMP in the regulation of developmental processes in Streptomyces.
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