Journal
JOURNAL OF BACTERIOLOGY
Volume 191, Issue 8, Pages 2675-2682Publisher
AMER SOC MICROBIOLOGY
DOI: 10.1128/JB.01814-08
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Funding
- U.S. Department of Energy (DOE)
- Virtual Institute for Microbial Stress and Survival
- Office of Science
- Office of Biological and Environmental Research
- GTL [DE-AC0205CH11231]
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Three mutants deficient in hydrogen/formate uptake were obtained through screening of a transposon mutant library containing 5,760 mutants of Desulfovibrio desulfuricans G20. Mutations were in the genes encoding the type I tetraheme cytochrome c(3) (cycA), Fe hydrogenase (hydB), and molybdopterin oxidoreductase (mopB). Mutations did not decrease the ability of cells to produce H-2 or formate during growth. Complementation of the cycA and mopB mutants with a plasmid carrying the intact cycA and/or mopB gene and the putative promoter from the parental strain allowed the recovery of H-2 uptake ability, showing that these specific genes are involved in H-2 oxidation. The mop operon encodes a periplasm-facing transmembrane protein complex which may shuttle electrons from periplasmic cytochrome c(3) to the menaquinone pool. Electrons can then be used for sulfate reduction in the cytoplasm.
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