Journal
NATURE REVIEWS MOLECULAR CELL BIOLOGY
Volume 16, Issue 9, Pages 568-576Publisher
NATURE PORTFOLIO
DOI: 10.1038/nrm4014
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Funding
- UK Engineering and Physical Research Council (EPSRC) [EP/J02175X/1]
- EU [KBBE.2011.5-289326]
- Marie Curie Intra-European Fellowship [628019]
- BBSRC [BB/K019791/1, BB/J020036/1, BB/M00550X/1] Funding Source: UKRI
- EPSRC [EP/J02175X/1, EP/G036004/1] Funding Source: UKRI
- Biotechnology and Biological Sciences Research Council [BB/K019791/1, BB/M00550X/1, BB/J020036/1] Funding Source: researchfish
- Engineering and Physical Sciences Research Council [EP/J02175X/1, EP/G036004/1] Funding Source: researchfish
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DNA assembly is a key part of constructing gene expression systems and even whole chromosomes. In the past decade, a plethora of powerful new DNA assembly methods - including Gibson Assembly, Golden Gate and ligase cycling reaction (LCR) - have been developed. In this Innovation article, we discuss these methods as well as standards such as the modular cloning (MoClo) system, GoldenBraid, modular overlap-directed assembly with linkers (MODAL) and PaperClip, which have been developed to facilitate a streamlined assembly workflow, to aid the exchange of material between research groups and to create modular reusable DNA parts.
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