Journal
JOURNAL OF ASSISTED REPRODUCTION AND GENETICS
Volume 30, Issue 11, Pages 1415-1419Publisher
SPRINGER/PLENUM PUBLISHERS
DOI: 10.1007/s10815-013-0086-y
Keywords
Cancer survivor; Cryopreserved sperm; Annexin V-MACS
Funding
- CIHR [SUR-103395, MOP-86636]
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Purpose The aim of the study is to report successful outcome (live births) after sperm sorting with annexin V-MACS on cryopreserved spermatozoa with high level of sperm DNA fragmentation from a cancer patient survivor. Methods Cryopreserved spermatozoa were sorted with annexin V-MACS prior to ICSI. Sperm DNA fragmentation was evaluated by SCSA((R)) and TUNEL. Results The couple had two previous IVF/ICSI cycles failures using sperm cryopreserved before cancer treatment. On third ICSI cycle attempt results were as follow: pre-annexin V-MACS sperm quality: 10 x 10(6)/ml, 3.3 % progressive motility, 1 % normal forms, TUNEL: 72.5 % positive cells, SCSA((R)): 76.6 % DFI. Post-annexin V-MACS sperm quality: 2.8 x 10(6)/ml, 10 % progressive motility, TUNEL: 58.8 % positive cells. Eight metaphase II oocytes were collected, 4 fertilized, 2 embryos were transferred on day 3 and healthy twins were born (1 boy, 1 girl). Conclusions Annexin V-MACS technique could be a potential tool to improve sperm quality on cryopreserved spermatozoa of cancer patient and improve ICSI outcome.
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