Journal
JOURNAL OF ASSISTED REPRODUCTION AND GENETICS
Volume 26, Issue 2-3, Pages 143-149Publisher
SPRINGER/PLENUM PUBLISHERS
DOI: 10.1007/s10815-009-9298-6
Keywords
Spermatogonia cells; Magnetic activated cell sorting; Cryopreservation; Infertility
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Isolating spermatogonia cells with high purity and viability and achieving better survival rate following cryopreservation Isolating the cells by Magnetic Activating Cell Sorting (MACS) method using anti CD49f (alpha 6 integrin) antibody and Dynabeads and freezing in DMSO-based freezing mediums containing three different FBS concentrations of 50%, 60% and 70%. The mean (+/- SD) purity of the isolated cells was 92.52 +/- 3.57 (range 92.43-98.25). The cells frozen in group I, II and III had mean 39.60 +/- 1.48 (range 37.98-41.62), 89.05 +/- 3.83 (range 80.83-90.33) and 90.52 +/- 1.71 (range 89.07-92.52) viability, respectively. Higher viable cell counts and purity can be attained by the use of alpha 6 integrin and magnetic beads. After the thawing of spermatogonial cells, optimum viability was achieved in freezing media containing 60% FBS.
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