Journal
JOURNAL OF ASSISTED REPRODUCTION AND GENETICS
Volume 26, Issue 11-12, Pages 629-635Publisher
SPRINGER/PLENUM PUBLISHERS
DOI: 10.1007/s10815-009-9369-8
Keywords
Conventional freezing; Vitrification; Human embryo biopsy; Implantation rate (IR); Pregnancy rate (PR)
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Cryopreservation of blastocysts, especially those subjected to the trauma due to blastomere biopsy for the purposes of pre-implantation genetic screening (PGS), requires significant optimization. Laboratory and clinical outcomes were compared to determine the effect of two different cryopreservation techniques on the development of human pre-implantation embryos that underwent blastomere biopsy and blastocoel drainage prior to cryopreservation. Retrospective clinical study. Women who requested cryotransfer of supernumerary blastocysts were analyzed by FISH. The main outcome measures were post-thaw survival (SR), pregnancy (PR), and implantation (IR). The SR of slowly frozen blastocysts was 83% compared to 97% for vitrified blastocysts. In 160 cases where biopsied embryos were cryotransferred, the results for slowly frozen versus vitrified blastocysts were: SR (71% vs. 95%), PR (23% vs. 37%), and IR (26% vs. 36%, P < 0.05), respectively. The results revealed that vitrified blastocysts provided higher SR, PR and IR as compared to slowly frozen counterparts.
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