Chemical composition and larvicidal activity of leaf essential oil from Clausena dentata (Willd) M. Roam. (Rutaceae) against the chikungunya vector, Aedes aegypti Linn. (Diptera: Culicidae)

Larvicidal activity of essential oil and isolated compounds from Clausena dentata leaves were tested against early fourth instar Aedes aegypti larvae. GC-MS analysis of essential oil revealed the presence of fourteen compounds of which the major compounds were sabinene (21.27%), biofloratriene (19.61%), borneol (18.34%) and beta-bisabolol (17.68%). The essential oil of C. dentata exhibited significant larvicidal activity, with 24 h LC50 and LC90 values of 140.2 and 341.6 mg/l, respectively. Larvicidal activities of the four major compounds of essential oil were also tested. The LC50 values of sabinene, biofloratriene, borneol and beta-bisabolol were 27.3, 47.4, 43.5 and 33.2 mg/l, respectively. Results of this study show that the leaf essential oil of C. dentata and its four major compounds may be a potent source of natural larvicides. (C) Korean Society of Applied Entomology, Taiwan Entomological Society and Malaysian Plant Protection Society, 2010 Published by Elsevier B.V. All rights reserved.