Journal
JOURNAL OF ARCHAEOLOGICAL SCIENCE
Volume 37, Issue 6, Pages 1269-1273Publisher
ACADEMIC PRESS LTD- ELSEVIER SCIENCE LTD
DOI: 10.1016/j.jas.2009.12.029
Keywords
Trichuris trichiura; Ancient DNA; Korea; PCR; Cloning and sequencing
Funding
- National Research Institute of Cultural Heritage of Cultural Heritage Administration [08D011Y-00110-2008]
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Despite high prevalence of Trichuris trichiura infection, PCR-based analysis on T trichiura from archaeological samples has not been established so far. In the present study, we sought to perform PCR-based amplification of T. trichiura aDNA using the sediments from medieval tomb of Korea. The presence of Trichuris eggs were first detected by microscopic observation; then confirmed by PCR-based aDNA analysis. Obtained sequence showed 100% homology to that of the small subunit ribosomal RNA (SSUrRNA) gene of T. trichiura but distinct from that of other Trichuris species. PCR-based aDNA analysis in this study can serve as effective method to confirm the presence of T trichiura eggs in the soils or coprolites collected from archaeological sites. (C) 2010 Elsevier Ltd. All rights reserved.
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