Journal
JOURNAL OF APPLIED TOXICOLOGY
Volume 33, Issue 8, Pages 838-844Publisher
WILEY
DOI: 10.1002/jat.2741
Keywords
cocaine; benzoylecgonine; cocaethylene; hair testing; GC; MS; interpretation of results
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A simple, rapid and sensitive method has been developed and validated for the determination of cocaine and its main metabolites (benzoylecgonine and cocaethylene) in human hair. The method involved solid-phase extraction with an Oasis HLB extraction cartridge and subsequent analysis by GC/MS. The limit of detection was 0.01ngmg-1 for cocaine, 0.04 for benzoylecgonine and 0.03 for cocaethylene. The method validation included linearity (with a correlation coefficient >0.99 over the range 0.2-50ngmg-1), intra- and inter-day precision (always lower than 12%) and accuracy (mean relative error always below 17%) to meet the bioanalytical acceptance criteria. The procedure was further applied to 40 hair samples from self-reported cocaine users arrested by the police who provided a positive urine-analysis for cocaine, and was demonstrated to be suitable for its application in forensic toxicology. New approaches were raised to detect false-negative results that allow a better interpretation of hair testing results. Copyright (c) 2012 John Wiley & Sons, Ltd.
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