Journal
JOURNAL OF APPLIED SPECTROSCOPY
Volume 85, Issue 3, Pages 573-579Publisher
SPRINGER
DOI: 10.1007/s10812-018-0688-3
Keywords
confocal Raman microscopy imaging; human skin tissue; Raman imaging; K-mean cluster analysis
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Funding
- Natural Science Foundation of China [11404258, 81401018]
- Education Department of Shaanxi, China [15JS102]
- Major Fundamental Research Program of Shaanxi Province, China [2016ZDJC-15]
- Northwest University, Shaanxi, China
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Confocal Raman microspectral imaging (CRMI) provides a versatile tool to extract biochemical information on histologically defined regions with improved spatial resolution and sensitivity. Here, we investigate spectral features and perform both uni- and multivariate analyses to obtain precise correlation between the biochemical profile and histological architecture of ex vivo human skin tissue. The skin dermis layer is visualized by integrated images of collagen peaks at 855, 940, and 1248 cm(-1), while the stratum corneum layer is described by chemical images of ceramides. M elanin accumulation patterns in epidermis are obtained by integration of 1375 and 1580 cm(-1) Raman peaks. In addition, distinct spectral features of the melanin precursor are revealed by certain Raman vibrations, which structurally characterize the molecular composition and organization of hair follicles. These results offer a unique insight into the skin histology, biochemistry, and biophysics, which provide the possibility of interpreting the underlying mechanisms of skin malignant processes.
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