Journal
NATURE METHODS
Volume 12, Issue 10, Pages 943-946Publisher
NATURE PUBLISHING GROUP
DOI: 10.1038/NMETH.3541
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Funding
- US National Institutes of Health (NIH) [T32GM008284]
- US NIH [GM082893, GM098672, GM082250, GM063210, 0D009180, GM110580]
- Phenix Industrial Consortium
- US Department of Energy [DE-AC02-05CH11231]
- Searle Scholar award from the Kinship Foundation
- Pew Scholar award from the Pew Charitable Trusts
- Packard Fellow award from the Lucille and David Packard Foundation
- US National Science Foundation [STC-1231306]
- UCSF-SABRE Innovation grant
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Advances in high-resolution cryo-electron microscopy (cryo-EM) require the development of validation metrics to independently assess map quality and model geometry. We report EMRinger, a tool that assesses the precise fitting of an atomic model into the map during refinement and shows how radiation damage alters scattering from negatively charged amino acids. EMRinger (https://github.com/fraser-lab/EMRinger) will be useful for monitoring progress in resolving and modeling high-resolution features in cryo-EM.
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