Journal
NATURE METHODS
Volume 12, Issue 8, Pages 733-U51Publisher
NATURE PUBLISHING GROUP
DOI: 10.1038/NMETH.3444
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Funding
- Medical Research Council
- UK National Institute for Health Research (NIHR) Surgical Reconstruction and Microbiology Research Centre
- Ontario Institute for Cancer Research through the Government of Ontario
- EU COST action [ES1103]
- MRC [MR/J014370/1] Funding Source: UKRI
- Medical Research Council [MR/M501621/1, MR/J014370/1] Funding Source: researchfish
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We have assembled de novo the Escherichia coli K-12 MG1655 chromosome in a single 4.6-Mb contig using only nanopore data. Our method has three stages: (i) overlaps are detected between reads and then corrected by a multiple-alignment process; (ii) corrected reads are assembled using the Celera Assembler; and (iii) the assembly is polished using a probabilistic model of the signal-level data. The assembly reconstructs gene order and has 99.5% nucleotide identity.
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