Journal
NATURE METHODS
Volume 12, Issue 10, Pages 927-930Publisher
NATURE PUBLISHING GROUP
DOI: 10.1038/NMETH.3554
Keywords
-
Categories
Funding
- US National Institute of Health NIDDK [R01DK101328]
- NHLBI [P01HL053750]
- NHGRI [U54HG007010]
Ask authors/readers for more resources
Regulatory regions harbor multiple transcription factor (TF) recognition sites; however, the contribution of individual sites to regulatory function remains challenging to define. We describe an approach that exploits the error-prone nature of genome editing-induced double-strand break repair to map functional elements within regulatory DNA at nucleotide resolution. We demonstrate the approach on a human erythroid enhancer, revealing single TF recognition sites that gate the majority of downstream regulatory function.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available