Journal
NATURE METHODS
Volume 12, Issue 4, Pages 335-U84Publisher
NATURE PUBLISHING GROUP
DOI: 10.1038/NMETH.3287
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Funding
- US National Institutes of Health [R01GM092802, EB001567, R01GM098672]
- Swiss systems biology initiative SystemsX.ch grant CINA
- University of California, San Francisco, Program for Breakthrough Biomedical Research
- Howard Hughes Medical Institute
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We present a de novo model-building approach that combines predicted backbone conformations with side-chain fit to density to accurately assign sequence into density maps. This method yielded accurate models for six of nine experimental maps at 3.3- to 4.8 resolution and produced a nearly complete model for an unsolved map containing a 660-residue heterodimeric protein. This method should enable rapid and reliable protein structure determination from near-atomic-resolution cryo-electron microscopy (cryo-EM) maps.
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