Journal
NATURE METHODS
Volume 12, Issue 11, Pages 1055-1057Publisher
NATURE PUBLISHING GROUP
DOI: 10.1038/NMETH.3590
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Funding
- Austrian Academy of Sciences
- European Union (FP7) [259348]
- Austrian Science Fund (FWF) [F4711]
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Thermal stabilization of proteins after ligand binding provides an efficient means to assess the binding of small molecules to proteins. We show here that in combination with quantitative mass spectrometry, the approach allows for the systematic survey of protein engagement by cellular metabolites and drugs. We profiled the targets of the drugs meth'otrexate and (S)-crizotinib and the metabolite 2'3'-cGAMP in intact cells and identified the 2'3'-cGAMP cognate transmembrane receptor STING, involved in immune signaling.
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