Journal
NATURE METHODS
Volume 12, Issue 11, Pages 1051-1054Publisher
NATURE PUBLISHING GROUP
DOI: 10.1038/NMETH.3580
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Funding
- US National Institutes of Health National Human Genome Research Institute [P50 HG005550]
- US Department of Energy [DE-FG02-02ER63445]
- Wyss Institute for Biologically Inspired Engineering
- US Army Research Office (DARPA) [W911NF-11-2-0054]
- National Science Foundation (Emerging Frontiers in Research and Innovation Award in Engineering New Technologies Based on Multicellutar and Inter-kingdom Signaling)
- US National Institutes of Health [5R01CA155320-04, P50 GM098792]
- National Cancer Institute [5T32CA009216-34]
- National Science Foundation Graduate Research Fellowship Program
- Department of Biological Engineering at MIT
- Department of Genetics at Harvard Medical School
- Canadian Institutes of Health Research
- Defense Threat Reduction Agency [HDTRA1-14-1-0006]
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We demonstrate that by altering the length of Cas9-associated guide RNA (gRNA) we were able to control Cas9 nuclease activity and simultaneously perform genome editing and transcriptional regulation with a single Cas9 protein. We exploited these principles to engineer mammalian synthetic circuits with combined transcriptional regulation and kill functions governed by a single multifunctional Cas9 protein.
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