Journal
NATURE METHODS
Volume 12, Issue 9, Pages 823-+Publisher
NATURE PUBLISHING GROUP
DOI: 10.1038/NMETH.3473
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Funding
- US National Institutes of Health [P50 HG005550]
- Banting Fellowship from the Canadian Institutes of Health Research
- University of California, San Diego
- Burroughs Wellcome Career Award at the Scientific Interface
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We developed an in vivo library-on-library methodology to simultaneously assess single guide RNA (sgRNA) activity across similar to 1,400 genomic loci. Assaying across multiple human cell types and end-processing enzymes as well as two Cas9 orthologs, we unraveled underlying nucleotide sequence and epigenetic parameters. Our results and software (http://crispr.med.harvard.edu/sgRNAScorer) enable improved design of reagents, shed light on mechanisms of genome targeting, and provide a generalizable framework to study nucleic acid-nucleic acid interactions and biochemistry in high throughput.
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